Establish of Protocol for Cucumis melo l. Using Different Hormones Through Plant Tissue Culture Technique

Authors

  • Ain Ullah Department of Botany University of Balochistan, Pakistan
  • Ayeesha Masood Department of Botany University of Balochistan, Pakistan
  • Basra Akhtar Department of Botany University of Balochistan, Pakistan
  • Bakht Zareen Rahim Department of Botany University of Balochistan, Pakistan
  • Ateeq Ullah Kakar Department of Botany University of Balochistan, Pakistan

DOI:

https://doi.org/10.5281/zenodo.8154898

Keywords:

Cucumis Melo L, MS Medium, Regeneration, Callogenesis, Organogenesis

Abstract

Melon (Cucumis melo L.) is economically importance plants in agricultural production in the province of Balochistan, Pakistan. In this study, we developed various regeneration and transformation protocols and we examined the different earlier morphological characters of Cucumis melo on MS medium with different combinations and concentration of plant growth regulators (PGRs) (Auxin, cytokinin, and gibberellin). Result showed that the maximum value of callus was obtained when 0.50mgl-1 of BAP was combined with 0.50mgl-1 of 2,4-D about 52%. The maximum regeneration percentage was obtained by the combination of 1mgl-1 NAA, 0.5 mgl-1 BAP and 0 mgl-1 KIN about 80%. Although, the mean number of shoots were displayed a maximum value in MS medium having the combination of 2.5mgl-1 NAA, 1mgl-1 BAP, and 0mgl-1 KIN about 2.4± 0.3. In addition, when the treatment was treated with 1.5mgl-1 BAP, and 0.5mgl-1 IBA showed a significant response on morphological parameters of studied plants. Similarly, the maximum number of roots, root and shoot length was observed when treated with 2 mgl-1 of IAA and IBA respectively. We assume that statement of the research findings presented here lead to for further studies on in vitro propagation of melon plants

 

 

 

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Author Biographies

Ain Ullah, Department of Botany University of Balochistan, Pakistan

 

 

Ayeesha Masood, Department of Botany University of Balochistan, Pakistan

 

 

Basra Akhtar, Department of Botany University of Balochistan, Pakistan

 

 

Bakht Zareen Rahim, Department of Botany University of Balochistan, Pakistan

 

 

Ateeq Ullah Kakar, Department of Botany University of Balochistan, Pakistan

 

 

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Published

2023-07-17
CITATION
DOI: 10.5281/zenodo.8154898
Published: 2023-07-17

How to Cite

Ain Ullah, Ayeesha Masood, Basra Akhtar, Bakht Zareen Rahim, & Ateeq Ullah Kakar. (2023). Establish of Protocol for Cucumis melo l. Using Different Hormones Through Plant Tissue Culture Technique. Pakistan’s Multidisciplinary Journal for Arts & Science, 4(02), 128–140. https://doi.org/10.5281/zenodo.8154898

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Articles